InhaltsangabeBasic Investigations in Saccharomyces cerevisiae



Brendan P. G. Curran and Virginia Bugeja



Isolation of Nucleic Acids



Michelle Hanna and Wei Xiao



Purification of Yeast Peroxisomes



Ben Distel and Astrid Kragt



Isolation of Yeast Plasma Membranes



Barry Panaretou and Peter Piper



Isolation of Yeast Mitochondria



Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott



Extraction of Yeast Lipids



Roger Schneiter and Günther Daum



TwoDimensional Gel Electrophoresis of Total Yeast Proteins



Hélian Boucherie and Christelle Monribot-Espagne



PulsedField Gel Electrophoresis of Budding Yeast Chromosomes



Laura Maringele and David Lydall. Analysis of Yeast Lipids, Roger Schneiter and Günther Daum



Yeast Fluorescence Microscopy



Jirí Hasek



Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe



Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, and Sean M. Hemmingsen



Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method



R. Daniel Gietz and Robin A. Woods



Mutagenesis



Leslie Barbour, Michelle Hanna, and Wei Xiao



Gene Disruption in the Budding Yeast Saccharomyces cerevisiae



Johannes H. Hegemann, Ulrich Güldener, and Gabriele J. Köhler



Inducible Degron and Its Application to Creating Conditional Mutants



R. Jürgen Dohmen



Synthetic Lethal Screen



Leslie Barbour and Wei Xiao



Synthetic Genetic Array Analysis in Saccharomyces cerevisiae



Amy Hin Yan Tong and Charles Boone



TwoDimensional Agarose Gel Analysis of DNA Replication Intermediates



Alain T. Dandjinou, Michel Larrivée, Ralf E. Wellinger, and Raymund J. Wellinger



Chromatin Assembly in a Crude Fraction From Yeast Cells



Karen M. Robinson and Michael C. Schultz



Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast



Elena Ezhkova and William P. Tansey



Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning



Zhengjian Zhang and Joseph C. Reese



Study of Transcriptional Regulation Using a Reporter Gene Assay



Yu Fu and Wei Xiao



Assessing Telomeric Phenotypes



Catherine LeBel, Michel Larrivée, Amadou Bah, Nancy Laterreur, Nancy Lévesque, and Raymund J. Wellinger



Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast



Zhigang Wang



Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier



Marja Makarow, Anna-Liisa Hänninen, Taina Suntio, and Ricardo Nunes Bastos



Yeast TwoHybrid System Screening



R. Daniel Gietz



Import of Precursor Proteins Into Isolated Yeast Mitochondria



Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling



Index