Brassica juncea (mustard) is one of the oil yielding and promising crop in India. The sub-optimal soil fertility is the main cause of low productivity of the cultivated lands. Nitrogen is the most important nutrient, which determines the growth of the mustard crop and increases the amount of protein and the yield. Phosphorus and potassium are known to be efficiently utilized in the presence of nitrogen. It promotes flowering, setting of silliqua and increase the size of silliqua and yield. The adequate and balanced supply of these plant nutrients is of critical importance in improving the productivity of oilseeds. Keeping above points in view, the present experiment was conducted to study the effect of different N Levels with and without P and K on the physico-chemical properties of soil and N, P and K availability in soil along with growth, yield and acquisition of nutrients by mustard.