Urinary tract infections (UTI) are major health problems affecting millions of people each year. As an opportunistic pathogen, Proteus mirabilis causes urinary tract infections. Ciprofloxacin is a recommended drug for the treatment of UTIs, but a progressive increase in fluoroquinolone resistance has been seen in clinical isolates. This study was a qualitative study, aimed to highlight the importance of using conventional and molecular techniques in the detection of different mutations within the genome of Proteus mirabilis isolates and their responsibility in fluoroquinolone.