InhaltsangabePart I: Detection, Quantification, and Confirmation1. Sampling the Production/Processing Environment for Listeria            Anca Ioana Nicolau and Andrei Sorin Bolocan2. Traditional Methods for Isolation of Listeria monocytogenes            Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, and Paula Teixeira3. Confirmation of Isolates of Listeria by Conventional and Real-Time PCR            David Rodríguez-Lázaro and Marta HernándezPart II: Characterization and Typing4. Serotype Assignment by Sero-Agglutination, ELISA, and PCR            Lisa Gorski5. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes            Marion Dalmasso and Kieran Jordan6. Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes            Beatrix Stessl, Irene Rückerl, and Martin Wagner7. Ribotyping and Automated Ribotyping of Listeria monocytogenes            Mazin Matloob and Mansel Griffiths8. Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes            Corinne Amar9. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray            Edward M. Fox and Kieran Jordan10. Analysis of Listeria monocytogenes Subproteomes            Michel Hébraud11. The Listeria Cell Wall and Associated Carbohydrate Polymers            Marcel R. Eugster and Martin J. Loessner12. Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria            Mathias Schmelcher and Martin J. Loessner13. Virulence Characterization of Listeria monocytogenes            Swetha Reddy and Mark L. Lawrence14. Internalization Assays for Listeria monocytogenes            Andreas Kühbacher, Pascale Cossart, and Javier Pizarro-CerdáPart III: Strain Manipulation15. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes            Aidan Casey and Olivia McAuliffe16. Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method            Kathrin Rychli, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter17. Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes            Reha Onur Azizoglu, Driss Elhanafi, and Sophia Kathariou18. Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR            Roberta Mazza and Rina Mazette19. Genome Sequencing of Listeria monocytogenes            Stephan Schmitz-Esser and Martin Wagner20. Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels            Marta Utratna and Conor P. O'ByrnePart IV: Control Methods21. Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers            Stavros G. Manios and Panagiotis N. Skandamis22. Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique            Mohammed Bahey-El-Din and Cormac G.M. Gahan23. Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments            Martin Wagner and Beatrix Stessl