Tomato is a candidate plant for complete genome sequencing, used as a part of functional genomic tools, such as insertional mutagenesis. EMS mutagenesis gives rise to a high mutation frequency without preference for specific genomic regions and generates many alleles that allow the isolation of several mutant phenotypes useful in research and crop breeding. The Book helps the readers in mutation related studies using EMS on Tomato seeds and its effect on HSPs & culturing under in vitro conditions via, plant tissue culture and also growth of tomato plant and obtaining healthy seeds for mutagenesis.