Cell and Tissue Culture Models in Dermatological Research


ISBN 9783642778193
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Inhaltsangabe1. Models in Orthology and Pathology.- Development of a Cell Culture System to Detect and Analyze Pathogenicity of Autoantibodies Directed to Nuclear Antigens.- Cell Cultures of Fetal Human Skin Are Suitable Models for the Investigation of Mitogenic Activity in Psoriasis Sera.- Influence of Human Sera on the Adherence of Candida albicans to Buccal and Intestinal Cells in Culture.- Modulation of 1,25-Dihydroxyvitamin D3 Receptor (VDR) Expression in HaCaT Keratinocytes.- Aminopeptidases of Human Skin - a Survey of Enzyme Activities in Extracts from Skin and Cultured Fibroblasts.- Determination of Cell Counts and Cell Proliferation by the Fluorochromes Hoechst 33342 or Hoechst 33258.- Volume Determination of Adhering Cells in Culture by Means of Acoustic Interferometry.- Effects of Uremic Compounds on Cultured Myocytes: a Model for Uremic Myocardiopathy.- Cultivation of Trophoblast Tissue of Human Intrauterine and Ectopic Pregnancies and Treatment with Methotrexate.- 2. Models in Differentiation of Keratinocytes.- Mechanical Stimulation Enhances Human Keratinocyte Differentiation in Culture: Induction of Cytokeratin 9 Synthesis.- N-Alkanes in Cultured Normal Human Keratinocytes.- Influence of Retinoids on the Keratin Profile in Normal and Spontaneously Transformed Human Keratinocytes.- Cytological Characterization of Three-dimensional, Graftable Human Keratinocytes Cultures.- Fluorometric Measurement of Suprabasal Cytokeratins and DNA of Human Keratinocytes Cultivated in Microtiter Plates.- 3. Models in Pigmentation.- Biochemistry of Mammalian Pigmentation: Enzymatic Regulation of Melanogenesis.- Cultured Epidermal Melanocytes from Patients with Neurofibromatosis 1 (Recklinghausen's Disease): a Cell Culture Model for Neurofibromatosis 1.- Pigmentation in Cultures of Human Melanocytes.- 4. Models in Wound Healing.- Re-epithelialization of Incision and Burn Wounds in Culture.- Primary Culture of Bilayered Epidermal Cell Sheets.- Plasminogen Activator Contained in Secretions of a Keratinocyte Cell Line and in the Fluid of Intraepidermal Pemphigus vulgaris Blisters Is Activated by Sodium dodecyl sulfate.- Are Rat Granulation Tissue Fibroblasts Regulated by Cytokines in the Same Manner as Skin Fibroblasts?.- Cultured Allografts for Wound Conditioning and Enhanced Epithelialization of Chronic Venous Ulcers.- Collagen Type-I Is Chemoattractive for the Human Keratinocyte Cell Line (HaCaT).- 5. Models in Fibroblast Function and Collagen Metabolism.- The Molecular Biology of the Terminal Differentiation of Dermal Fibroblasts in the Fibroblast Stem Cell System In Vivo and In Vitro.- Cytokine-dependent Regulation of Human Dermal Fibroblasts Cultured in a Three-dimensional Collagen-Gel.- Investigation of Components of the Dermal-Epidermal Junction Zone In Vitro: Effect of Transforming Growth Factor-beta on Collagen VII Expression.- The Influence of Retinoids on Chemotaxis and Connective Tissue Synthesis of Fibroblasts.- 6. Models in Skin Ageing.- In Vitro Studies of Fibroblast Aging: Growth Factors, Collagen and Glycosaminoglycan Synthesis - a Review.- Terminal Differentiation, Aging, Apoptosis, or Transformation of the WI-38 Fibroblasts in the Fibroblast Stem Cell System In Vitro.- Differential Gene Expression, Protein Synthesis, and Degradation in Aging Fibroblasts.- Age Differences in Human Skin Collagen.- Influence of Cell Density of Energy Metabolism and the Actin Skeleton of Different Cell Types.- 7. Models in Skin Pharmacology and Toxicology.- (+)-Aeroplysinin-l: an Inhibitor or Intrinsic Protein Tyrosine Kinase Activity of EGF-Receptor-Kinase Complex.- In Vitro Cultivation of Human Dermal Microvascular Endothelial Cells as a Model for the Dermatopharmacology.- Effect of Nicotine on the Differentiation of Human Keratinocytes In Vitro.- A New In Vitro Test System and Phototoxicological Studies on Cell Cultures Based on the Melamine Substrate Foil.- In Vitro Assays for Evaluation of the Effects of 8-Methoxypsoralen and UV Irradiation of
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