This book approaches biofuels from a new biotechnological perspective with the aim of equipping researchers with a thorough, up-to-date understanding of Biofuel research and related technologies. Topics include biomethane, bioethanol, biobutanol, biomass, hydrolysis, bio-oil, microbiology, and many more. Biofuel research have evolved mechanisms to advance its utilization and their application in our system. The chapters will provide concise and up-to-date information to aid our understandings on biotechnology related implications of biofuels development and its advancements. Readers in the areas of bioengineering, sustainability, renewable energies and biotechnology will find this work useful to widen their knowledge and perspectives on biofuels.