Plant tissue culture technique has opened new vistas not only in commercial propagation and crop improvement programme but plays a vital role in resolving mysteries of mechanism which are not possible in the intact plants due to complexity in their organisation. Identification of protein(s) synthesized during sequential stages of differentiation gives an insight into biochemical changes associated with differentiation. This master dissertation is divided into six chapters.Chapter-1 highlights the significance of the study along with objectives.Chapter-2 is compilation of relevant literature indicating the present status of the problem. Chapter-3 deals with the detailed account of materials and technique applied. Chapter-4 results along with tables, photographs and illustrations have been described. Chapter-5 highlights the results and their interpretation in the light of available literature. Chapter-6 presents summary of the work. In the last, literature cited has been given. This master dissertation will especially prove a boon and valuable asset for the post-graduate students specializing in the field of micropropagation, differentiation and its biochemical basis.