In this textbook, I described the techniques of molecular isolation and immunogenicity of Eimeria brunetti genes. The book composes 7 chapters such as: chapter 1 - Chicken Coccidiosis and Immune Response to Eimeria; Chapter 2 - Control of Chicken Coccidiosis; Chapter 3 - Isolation and Identification of Eimeria brunetti strain; Chapter 4 - Cloning, Expression and Characterization of Eimeria brunetti Microneme-2 Protein (EbMIC2); Chapter 5 - Immunogenicity of Eimeria brunetti Microneme-2 Protein and Its Protective Effects on Chicken; Chapter 6 - Cloning, Expression and Characterization of Eimeria brunetti Apical Membrane Antigen-1 (EbAMA1) and Chapter 7 - Immunogenicity of Eimeria brunetti Apical Membrane Antigen-1 and Its Protective Effects on Chicken.