We are living in an age of Science and Technology where man has reached to moon and has made remarkable achievements in various scientific spheres. A number of highly complicated and technical instruments have been devised to enable the modern researcher to evolve new ideas as theories. The new technology and instruments are an indispensable companion of a studying students and growing biologists. The fast changing face of Biotechnology has created a gap and has given impetus to the author present the Text Book of Biotechnology for the young students preparing for the degree courses.